Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Bordbar, AarashJohansson, Pär I.
Paglia, Giuseppe
Harrison, Scott J.
Wichuk, Kristine
Magnusdottir, Manuela
Valgeirsdottir, Sóley
Gybel-Brask, Mikkel
Ostrowski, Sisse R.
Palsson, Sirus
Rolfsson, Ottar
Sigurjónsson, Olafur E.
Hansen, Morten B.
Gudmundsson, Sveinn
Palsson, Bernhard O.
Issue Date
2016-04
Metadata
Show full item recordCitation
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes 2016, 56 (4):852 TransfusionAbstract
BACKGROUNDThere has been interest in determining whether older red blood cell (RBC) units have negative clinical effects. Numerous observational studies have shown that older RBC units are an independent factor for patient mortality. However, recently published randomized clinical trials have shown no difference of clinical outcome for patients receiving old or fresh RBCs. An overlooked but essential issue in assessing RBC unit quality and ultimately designing the necessary clinical trials is a metric for what constitutes an old or fresh RBC unit. STUDY DESIGN AND METHODSTwenty RBC units were profiled using quantitative metabolomics over 42 days of storage in SAGM with 3- to 4-day time intervals. Metabolic pathway usage during storage was assessed using systems biology methods. The detected time intervals of the metabolic states were compared to clinical outcomes. RESULTSUsing multivariate statistics, we identified a nonlinear decay process exhibiting three distinct metabolic states (Days 0-10, 10-17, and 17-42). Hematologic variables traditionally measured in the transfusion setting (e.g., pH, hemolysis, RBC indices) did not distinguish these three states. Systemic changes in pathway usage occurred between the three states, with key pathways changing in both magnitude and direction. Finally, an association was found between the time periods of the metabolic states with the clinical outcomes of more than 280,000 patients in the country of Denmark transfused over the past 15 years and endothelial damage markers in healthy volunteers undergoing autologous transfusions. CONCLUSIONThe state of RBC metabolism may be a better indicator of cellular quality than traditional hematologic variables.Description
To access publisher's full text version of this article click on the hyperlink at the bottom of the pageAdditional Links
http://doi.wiley.com/10.1111/trf.13460http://onlinelibrary.wiley.com/doi/10.1111/trf.13460/epdf
Rights
Archived with thanks to Transfusionae974a485f413a2113503eed53cd6c53
10.1111/trf.13460
Scopus Count