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dc.contributor.authorFreysdottir, Jona
dc.contributor.authorLogadottir, Oddny T
dc.contributor.authorOmarsdottir, Sesselja S
dc.contributor.authorVikingsson, Arnor
dc.contributor.authorHardardottir, Ingibjorg
dc.date.accessioned2016-06-16T16:05:43Zen
dc.date.available2016-06-16T16:05:43Zen
dc.date.issued2016-06-05en
dc.date.submitted2016en
dc.identifier.citationA polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes. 2016, 143:131-8 Carbohydr Polymen
dc.identifier.issn1879-1344en
dc.identifier.pmid27083352en
dc.identifier.doi10.1016/j.carbpol.2016.02.017en
dc.identifier.urihttp://hdl.handle.net/2336/613367en
dc.descriptionTo access publisher's full text version of this article click on the hyperlink at the bottom of the pageen
dc.description.abstractAchillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7. THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d. However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined. Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d. These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
dc.description.sponsorshipIcelandic Research Fund/110418021 University of Iceland Research Fund Landspitali University Hospital Research Funden
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://dx.doi.org/ 10.1016/j.carbpol.2016.02.017en
dc.rightsArchived with thanks to Carbohydrate polymersen
dc.subjectNAF12
dc.subjectRHE12
dc.subject.meshAchilleaen
dc.subject.meshCytokinesen
dc.subject.meshMAP Kinase Kinase Kinasesen
dc.subject.meshPolysaccharidesen
dc.titleA polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.en
dc.typeArticleen
dc.contributor.department[ 1 ] Landspitali, Ctr Rheumatol Res, IS-101 Reykjavik, Iceland [ 2 ] Univ Iceland, Biomed Ctr, Fac Med, Dept Immunol, IS-101 Reykjavik, Iceland [ 3 ] Landspitali, Dept Immunol, IS-101 Reykjavik, Iceland [ 4 ] Univ Iceland, Biomed Ctr, Fac Med, Dept Biochem & Mol Biol, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland [ 5 ] Univ Iceland, Fac Pharmaceut Sci, IS-107 Reykjavik, Icelanden
dc.identifier.journalCarbohydrate polymersen
dc.rights.accessNational Consortium - Landsaðganguren
html.description.abstractAchillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7. THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d. However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined. Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d. These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.


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