Molecular diagnosis of Legionella infections--Clinical utility of front-line screening as part of a pneumonia diagnostic algorithm.
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Gadsby, Naomi JHelgason, Kristjan O
Dickson, Elizabeth M
Mills, Jonathan M
Lindsay, Diane S J
Edwards, Giles F
Hanson, Mary F
Templeton, Kate E
Issue Date
2016-02
Metadata
Show full item recordCitation
Molecular diagnosis of Legionella infections--Clinical utility of front-line screening as part of a pneumonia diagnostic algorithm. 2016, 72 (2):161-70 J. Infect.Abstract
Urinary antigen testing for Legionella pneumophila serogroup 1 is the leading rapid diagnostic test for Legionnaires' Disease (LD); however other Legionella species and serogroups can also cause LD. The aim was to determine the utility of front-line L. pneumophila and Legionella species PCR in a severe respiratory infection algorithm.L. pneumophila and Legionella species duplex real-time PCR was carried out on 1944 specimens from hospitalised patients over a 4 year period in Edinburgh, UK.
L. pneumophila was detected by PCR in 49 (2.7%) specimens from 36 patients. During a LD outbreak, combined L. pneumophila respiratory PCR and urinary antigen testing had optimal sensitivity and specificity (92.6% and 98.3% respectively) for the detection of confirmed cases. Legionella species was detected by PCR in 16 (0.9%) specimens from 10 patients. The 5 confirmed and 1 probable cases of Legionella longbeachae LD were both PCR and antibody positive.
Front-line L. pneumophila and Legionella species PCR is a valuable addition to urinary antigen testing as part of a well-defined algorithm. Cases of LD due to L. longbeachae might be considered laboratory-confirmed when there is a positive Legionella species PCR result and detection of L. longbeachae specific antibody response.
Description
To access publisher's full text version of this article click on the hyperlink at the bottom of the pageAdditional Links
http://dx.doi.org/ 10.1016/j.jinf.2015.10.011Rights
Archived with thanks to The Journal of infectionae974a485f413a2113503eed53cd6c53
10.1016/j.jinf.2015.10.011
Scopus Count
Collections
Related articles
- Two years' performance of an in-house ELISA for diagnosis of Legionnaires' disease: detection of specific IgM and IgG antibodies against Legionella pneumophila serogroup 1, 3 and 6 in human serum.
- Authors: Elverdal PL, Jørgensen CS, Krogfelt KA, Uldum SA
- Issue date: 2013 Aug
- Travel-associated infections caused by unusual serogroups of Legionella pneumophila identified using Legionella BIOCHIP slides in Turkey and Iraq.
- Authors: Kocazeybek BS, Yuksel P, Keskin D, Sheikh S, Habip Z, Yavuzer SS, Caliskan R, Altun YM, Kuskucu M, Cengiz M, Dinc HO, Karakullukcu A, Ergin S, Saribas S, Yilmaz N, Tokman HB
- Issue date: 2016 May-Jun
- Legionnaires' disease caused by Legionella longbeachae: Clinical features and outcomes of 107 cases from an endemic area.
- Authors: Isenman HL, Chambers ST, Pithie AD, MacDonald SL, Hegarty JM, Fenwick JL, Maze MJ, Metcalf SC, Murdoch DR
- Issue date: 2016 Oct
- Genetic susceptibility and caspase activation in mouse and human macrophages are distinct for Legionella longbeachae and L. pneumophila.
- Authors: Asare R, Santic M, Gobin I, Doric M, Suttles J, Graham JE, Price CD, Abu Kwaik Y
- Issue date: 2007 Apr
- Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples.
- Authors: Nazarian EJ, Bopp DJ, Saylors A, Limberger RJ, Musser KA
- Issue date: 2008 Oct