Show simple item record

dc.contributor.authorRolfsson, Óttar
dc.contributor.authorJohannsson, Freyr
dc.contributor.authorMagnusdottir, Manuela
dc.contributor.authorPaglia, Giuseppe
dc.contributor.authorSigurjonsson, Ólafur E.
dc.contributor.authorBordbar, Aarash
dc.contributor.authorPalsson, Sirus
dc.contributor.authorBrynjólfsson, Sigurður
dc.contributor.authorGuðmundsson, Sveinn
dc.contributor.authorPalsson, Bernhard
dc.date.accessioned2017-11-24T14:09:27Z
dc.date.available2017-11-24T14:09:27Z
dc.date.issued2017-11
dc.date.submitted2017
dc.identifier.citationMannose and fructose metabolism in red blood cells during cold storage in SAGM 2017, 57 (11):2665 Transfusionen
dc.identifier.issn00411132
dc.identifier.doi10.1111/trf.14266
dc.identifier.urihttp://hdl.handle.net/2336/620363
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen
dc.description.abstractBACKGROUND: Alternate sugar metabolism during red blood cell (RBC) storage is not well understood. Here we report fructose and mannose metabolism in RBCs during cold storage in SAGM and the impact that these monosaccharides have on metabolic biomarkers of RBC storage lesion. STUDY DESIGN AND METHODS: RBCs were stored in SAGM containing uniformly labeled 13 C-fructose or 13 C-mannose at 9 or 18 mmol/L concentration for 25 days. RBCs and media were sampled at 14 time points during storage and analyzed using ultraperformance liquid chromatography-mass spectrometry. Blood banking quality assurance measurements were performed. RESULTS: Red blood cells incorporated fructose and mannose during cold storage in the presence of glucose. Mannose was metabolized in preference to glucose via glycolysis. Fructose lowered adenosine triphosphate (ATP) levels and contributed little to ATP maintenance when added to SAGM. Both monosaccharides form the advanced glycation end product glycerate. Mannose activates enzymes in the RBC that take part in glycan synthesis. CONCLUSIONS: Fructose or mannose addition to RBC SAGM concentrates may not offset the shift in metabolism of RBCs that occurs after 10 days of storage. Fructose and mannose metabolism at 4°C in SAGM reflects their metabolism at physiologic temperature. Glycerate excretion is a measure of protein deglycosylation activity in stored RBCs. No cytoprotective effect was observed upon the addition of either fructose or mannose to SAGM.
dc.description.sponsorshipEuropean Research Council RANNIS Granten
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://doi.wiley.com/10.1111/trf.14266en
dc.rightsArchived with thanks to Transfusionen
dc.subjectBlóðkornen
dc.subjectEfnahvörfen
dc.subjectNAF12en
dc.subjectBAB12en
dc.subject.meshBlood Preservationen
dc.subject.meshMannoseen
dc.subject.meshFructoseen
dc.subject.meshErythrocytesen
dc.titleMannose and fructose metabolism in red blood cells during cold storage in SAGMen
dc.typeArticleen
dc.contributor.department[ 1 ] Univ Iceland, Ctr Syst Biol, Sturlugata 8, Reykjavik, Iceland [ 2 ] Univ Iceland, Dept Med, Reykjavik, Iceland [ 3 ] Landspitali Univ Hosp, Blood Bank, Reykjavik, Iceland [ 4 ] Reykjavik Univ, Sch Sci & Engn, Reykjavik, Iceland [ 5 ] European Acad Bolzano Bozen, Ctr Biomed, Bolzano, Italy [ 6 ] Sinopia Biosci, San Diego, CA USAen
dc.identifier.journalTransfusionen
dc.rights.accessNational Consortium - Landsaðganguren
dc.contributor.institutionCenter for Systems Biology
dc.contributor.institutionCenter for Systems Biology
dc.contributor.institutionCenter for Systems Biology
dc.contributor.institutionCenter for Systems Biology
dc.contributor.institutionThe Blood Bank, Landspitali-University Hospital
dc.contributor.institutionSinopia Bioscience; San Diego California
dc.contributor.institutionSinopia Bioscience; San Diego California
dc.contributor.institutionCenter for Systems Biology
dc.contributor.institutionThe Blood Bank, Landspitali-University Hospital
dc.contributor.institutionCenter for Systems Biology
html.description.abstractBACKGROUND: Alternate sugar metabolism during red blood cell (RBC) storage is not well understood. Here we report fructose and mannose metabolism in RBCs during cold storage in SAGM and the impact that these monosaccharides have on metabolic biomarkers of RBC storage lesion. STUDY DESIGN AND METHODS: RBCs were stored in SAGM containing uniformly labeled 13 C-fructose or 13 C-mannose at 9 or 18 mmol/L concentration for 25 days. RBCs and media were sampled at 14 time points during storage and analyzed using ultraperformance liquid chromatography-mass spectrometry. Blood banking quality assurance measurements were performed. RESULTS: Red blood cells incorporated fructose and mannose during cold storage in the presence of glucose. Mannose was metabolized in preference to glucose via glycolysis. Fructose lowered adenosine triphosphate (ATP) levels and contributed little to ATP maintenance when added to SAGM. Both monosaccharides form the advanced glycation end product glycerate. Mannose activates enzymes in the RBC that take part in glycan synthesis. CONCLUSIONS: Fructose or mannose addition to RBC SAGM concentrates may not offset the shift in metabolism of RBCs that occurs after 10 days of storage. Fructose and mannose metabolism at 4°C in SAGM reflects their metabolism at physiologic temperature. Glycerate excretion is a measure of protein deglycosylation activity in stored RBCs. No cytoprotective effect was observed upon the addition of either fructose or mannose to SAGM.


This item appears in the following Collection(s)

Show simple item record