Show simple item record

dc.contributor.authorMagdalena M.Stefaniak-Vidarssona, Varsha A.Kaleb, María Guðjónsdóttir, Guðrún Marteinsdottir, Ólafur Friðjónsson, Gudmundur Óli Hreggvidsson, Ólafur E.Sigurjónsson, Sesselja Ómarsdottir, Kristberg Kristbergsson
dc.date.accessioned2018-03-27T10:40:42Z
dc.date.available2018-03-27T10:40:42Z
dc.date.issued2017-09-21
dc.date.submitted2018
dc.identifier.citationBioactive Carbohydrates and Dietary Fibre 2017,12:14-19en
dc.identifier.issn2212-6198
dc.identifier.urihttp://hdl.handle.net/2336/620523
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen
dc.description.abstractAtlantic sea cucumber (Cucumaria frondosa) is a marine invertebrate that occurs naturally in the North Atlantic. Its collagen rich body walls make the sea cucumber an appreciated food source in many countries. The heteroglycans derived from the cartilage of Atlantic sea cucumbers have shown potential as a source of bioactive compounds, but further investigation is needed to confirm this hypothesis. The objectives of this work were to test the immunomodulating properties of heteroglycan fractions (FCFs) derived from Atlantic sea cucumbers toward phorbol 12-myristate 13-acetate (PMA) differentiated THP-1 macrophages. Oxygen Radical Absorbance Capacity (ORAC) was determined and cell culture media was collected and analyzed for presence of pro-inflammatory and anti-inflammatory cytokines (TNF-α, IL-6, and IL-10). FCFs toxicity toward macrophages was evaluated by light microscopy and confirmed by XTT proliferation assay. Heteroglycans derived from cartilage of Atlantic sea cucumber showed immunomodulatory activity in THP-1 macrophages. FCF-1, −2, and − 3) at concentrations 0.1–100 µg/mL induced levels of TNF-α, IL-6, and IL-10 proofing their bioactivity towards THP-1 macrophages. However, fractions of FCF-1 and FCF-2 may have induced oxidative stress in the macrophages, which possibly could lead to decreased cellular viability.
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://ac.els-cdn.com/S221261981730027X/1-s2.0-S221261981730027X-main.pdf?_tid=64258614-35ff-4f7e-8fe6-eb188c0d6098&acdnat=1522146102_20228481004fc92fc90d2ea1c8a3b66ben
dc.subjectSjávarlíffræðien
dc.subjectBAB12en
dc.subject.meshSea Cucumbersen
dc.subject.meshPolysaccharidesen
dc.subject.meshMacrophagesen
dc.titleBioactive effect of sulphated polysaccharides derived from orange-footed sea cucumber (Cucumaria frondosa) toward THP-1 macrophagesen
dc.typeArticleen
dc.contributor.departmenta Faculty of Food Science and Nutrition, School of Health Sciences, University of Iceland, Iceland b Matis ohf., Reykjavik, Iceland c School of Engineering and Natural Sciences, University of Iceland, Iceland d Tara Mar ehf, Reykjavik, Iceland e Blood Bank, Landspitali University Hospital, Reykjavik, Iceland f School of Science and Engineering, Reykjavik University, Reykjavik, Iceland g Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Icelanden
dc.identifier.journalBioactive Carbohydrates and Dietary Fibreen
dc.rights.accessNational Consortium - Landsaðganguren
dc.departmentcodeBAB12
html.description.abstractAtlantic sea cucumber (Cucumaria frondosa) is a marine invertebrate that occurs naturally in the North Atlantic. Its collagen rich body walls make the sea cucumber an appreciated food source in many countries. The heteroglycans derived from the cartilage of Atlantic sea cucumbers have shown potential as a source of bioactive compounds, but further investigation is needed to confirm this hypothesis. The objectives of this work were to test the immunomodulating properties of heteroglycan fractions (FCFs) derived from Atlantic sea cucumbers toward phorbol 12-myristate 13-acetate (PMA) differentiated THP-1 macrophages. Oxygen Radical Absorbance Capacity (ORAC) was determined and cell culture media was collected and analyzed for presence of pro-inflammatory and anti-inflammatory cytokines (TNF-α, IL-6, and IL-10). FCFs toxicity toward macrophages was evaluated by light microscopy and confirmed by XTT proliferation assay. Heteroglycans derived from cartilage of Atlantic sea cucumber showed immunomodulatory activity in THP-1 macrophages. FCF-1, −2, and − 3) at concentrations 0.1–100 µg/mL induced levels of TNF-α, IL-6, and IL-10 proofing their bioactivity towards THP-1 macrophages. However, fractions of FCF-1 and FCF-2 may have induced oxidative stress in the macrophages, which possibly could lead to decreased cellular viability.


This item appears in the following Collection(s)

Show simple item record