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Northern lights assay: a versatile method for comprehensive detection of DNA damage.

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Authors
Gudmundsson, Bjarki
Thormar, Hans G
Sigurdsson, Albert
Dankers, Wendy
Steinarsdottir, Margret
Hermanowicz, Stefan
Sigurdsson, Stefan
Olafsson, David
Halldorsdottir, Anna M
Meyn, Stephen
Jonsson, Jon J
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Issue Date
2018-11-16

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Northern lights assay: a versatile method for comprehensive detection of DNA damage. 2018, 46(20):e118 Nucleic Acids Res
Abstract
DNA damage assays have various limitations in types of lesions detected, sensitivity, specificity and samples that can be analyzed. The Northern Lights Assay (NLA) is based on 2D Strandness-Dependent Electrophoresis (2D-SDE), a technique that separates nucleic acids based on length, strandness, structure and conformation changes induced by damage. NLA is run on a microgel platform in 20-25 min. Each specimen is analyzed in pairs of non-digested DNA to detect single- and double-stranded breaks (DSBs) and Mbo I-digested DNA to detect other lesions. We used NLA to evaluate DNA in solution and isolated from human cells treated with various genotoxic agents. NLA detected and distinguished between single- and DSBs, interstrand and intrastrand DNA crosslinks, and denatured single-stranded DNA. NLA was sufficiently sensitive to detect biologically relevant amount of DNA damage. NLA is a versatile, sensitive and simple method for comprehensive and simultaneous analysis of multiple types of damage, both in purified DNA and in DNA isolated from cells and body fluids. NLA can be used to evaluate DNA quality in biosamples, monitor complex molecular procedures, assess genotoxicity, diagnose genome instability, facilitate cancer theranostics and in basic nucleic acids research.
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https://academic.oup.com/nar/article/46/20/e118/5057061
ae974a485f413a2113503eed53cd6c53
10.1093/nar/gky645
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