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dc.contributor.authorGudmundsson, Bjarki
dc.contributor.authorThormar, Hans G
dc.contributor.authorSigurdsson, Albert
dc.contributor.authorDankers, Wendy
dc.contributor.authorSteinarsdottir, Margret
dc.contributor.authorHermanowicz, Stefan
dc.contributor.authorSigurdsson, Stefan
dc.contributor.authorOlafsson, David
dc.contributor.authorHalldorsdottir, Anna M
dc.contributor.authorMeyn, Stephen
dc.contributor.authorJonsson, Jon J
dc.date.accessioned2019-03-29T10:52:39Z
dc.date.available2019-03-29T10:52:39Z
dc.date.issued2018-11-16
dc.date.submitted2019-03
dc.identifier.citationNorthern lights assay: a versatile method for comprehensive detection of DNA damage. 2018, 46(20):e118 Nucleic Acids Resen_US
dc.identifier.issn1362-4962
dc.identifier.pmid30053193
dc.identifier.doi10.1093/nar/gky645
dc.identifier.urihttp://hdl.handle.net/2336/620848
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Downloaden_US
dc.description.abstractDNA damage assays have various limitations in types of lesions detected, sensitivity, specificity and samples that can be analyzed. The Northern Lights Assay (NLA) is based on 2D Strandness-Dependent Electrophoresis (2D-SDE), a technique that separates nucleic acids based on length, strandness, structure and conformation changes induced by damage. NLA is run on a microgel platform in 20-25 min. Each specimen is analyzed in pairs of non-digested DNA to detect single- and double-stranded breaks (DSBs) and Mbo I-digested DNA to detect other lesions. We used NLA to evaluate DNA in solution and isolated from human cells treated with various genotoxic agents. NLA detected and distinguished between single- and DSBs, interstrand and intrastrand DNA crosslinks, and denatured single-stranded DNA. NLA was sufficiently sensitive to detect biologically relevant amount of DNA damage. NLA is a versatile, sensitive and simple method for comprehensive and simultaneous analysis of multiple types of damage, both in purified DNA and in DNA isolated from cells and body fluids. NLA can be used to evaluate DNA quality in biosamples, monitor complex molecular procedures, assess genotoxicity, diagnose genome instability, facilitate cancer theranostics and in basic nucleic acids research.en_US
dc.description.sponsorshipUniversity of Iceland Research Fund Landspitali University Hospital Research Fund Icelandic Center for Research Funds Lifeind ehf. University of Iceland Research Funden_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.urlhttps://academic.oup.com/nar/article/46/20/e118/5057061en_US
dc.subjectDNA-rannsókniren_US
dc.subject.meshComet Assayen_US
dc.subject.meshDNA Damageen_US
dc.titleNorthern lights assay: a versatile method for comprehensive detection of DNA damage.en_US
dc.typeArticleen_US
dc.contributor.department1 Department of Biochemistry and Molecular Biology, University of Iceland, Reykjavik IS-101, Iceland. 2 Department of Genetics and Molecular Medicine, Landspitali-National University Hospital, Reykjavik IS-101, Iceland. 3 Lifeind ehf., Reykjavik IS-101, Iceland. 4 Faculty of Medicine, University of Iceland, Reykjavik IS-101, Iceland. 5 The Blood Bank, Landspitali-National University Hospital, Reykjavik IS-101, Iceland. 6 Department of Paediatrics, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada. 7 University of Toronto, Toronto, ON, M5S 1A8, Canada. 8 Center for Human Genomics and Precision Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53705, USA.en_US
dc.identifier.journalNucleic acids researchen_US
dc.rights.accessOpen Access - Opinn aðganguren_US
dc.departmentcodeNAF12
dc.departmentcodeBAB12
dc.source.journaltitleNucleic acids research
refterms.dateFOA2019-03-29T10:52:40Z


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