• English
    • íslenska
  • English 
    • English
    • íslenska
  • Login
View Item 
  •   Home
  • Journal Articles, Peer Reviewed (Ritrýndar vísindagreinar)
  • English Journal Articles (Peer Reviewed)
  • View Item
  •   Home
  • Journal Articles, Peer Reviewed (Ritrýndar vísindagreinar)
  • English Journal Articles (Peer Reviewed)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of HirslaCommunitiesAuthorsTitleSubjectsSubject (MeSH)Issue DateJournalThis CollectionAuthorsTitleSubjectsSubject (MeSH)Issue DateJournal

My Account

LoginRegister

Local Links

FAQ - (Icelandic)FAQ - (English)Hirsla LogosAbout LandspitaliLSH Home PageLibrary HomeIcelandic Journals

Statistics

Display statistics

Metabolic fate of adenine in red blood cells during storage in SAGM solution.

  • CSV
  • RefMan
  • EndNote
  • BibTex
  • RefWorks
Average rating
 
   votes
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
 
Your vote was cast
Thank you for your feedback
Authors
Paglia, Giuseppe
Sigurjónsson, Ólafur E
Bordbar, Aarash
Rolfsson, Óttar
Magnusdottir, Manuela
Palsson, Sirus
Wichuk, Kristine
Gudmundsson, Sveinn
Palsson, Bernhard O
Issue Date
2016-10

Metadata
Show full item record
Citation
Paglia G, Sigurjónsson ÓE, Bordbar A, Rolfsson Ó, Magnusdottir M, Palsson S, Wichuk K, Gudmundsson S, Palsson BO. Metabolic fate of adenine in red blood cells during storage in SAGM solution. Transfusion. 2016 Oct;56(10):2538-47.
Abstract
BACKGROUND: Red blood cells (RBCs) are routinely stored and transfused worldwide. Recently, metabolomics have shown that RBCs experience a three-phase metabolic decay process during storage, resulting in the definition of three distinct metabolic phenotypes, occurring between Days 1 and 10, 11 and 17, and 18 and 46. Here we use metabolomics and stable isotope labeling analysis to study adenine metabolism in RBCs. STUDY DESIGN AND METHODS: A total of 6 units were prepared in SAGM or modified additive solutions (ASs) containing 15 N5 -adenine. Three of them were spiked with 15 N5 -adenine on Days 10, 14, and 17 during storage. Each unit was sampled 10 times spanning Day 1 to Day 32. At each time point metabolic profiling was performed. RESULTS: We increased adenine concentration in the AS and we pulsed the adenine concentration during storage and found that in both cases the RBCs' main metabolic pathways were not affected. Our data clearly show that RBCs cannot consume adenine after 18 days of storage, even if it is still present in the storage solution. However, increased levels of adenine influenced S-adenosylmethionine metabolism. CONCLUSION: In this work, we have studied in detail the metabolic fate of adenine during RBC storage in SAGM. Adenine is one of the main substrates used by RBCs, but the metabolic shift observed during storage is not caused by an absence of adenine later in storage. The rate of adenine consumption strongly correlated with duration of storage but not with the amount of adenine present in the AS.
Description
To access publisher's full text version of this article click on the hyperlink below
Additional Links
https://onlinelibrary.wiley.com/doi/full/10.1111/trf.13740
https://ovidsp.dc1.ovid.com/sp-4.04.0a/ovidweb.cgi?QS2=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
Rights
© 2016 AABB.
ae974a485f413a2113503eed53cd6c53
10.1111/trf.13740
Scopus Count
Collections
English Journal Articles (Peer Reviewed)

entitlement

Related articles

  • Metabolomics of ADSOL (AS-1) red blood cell storage.
  • Authors: Roback JD, Josephson CD, Waller EK, Newman JL, Karatela S, Uppal K, Jones DP, Zimring JC, Dumont LJ
  • Issue date: 2014 Apr
  • Prevention of red cell storage lesion: a comparison of five different additive solutions.
  • Authors: Lagerberg JW, Korsten H, Van Der Meer PF, De Korte D
  • Issue date: 2017 Sep
  • Modified formulation of CPDA for storage of whole blood, and of SAGM for storage of red blood cells, to maintain the concentration of 2,3-diphosphoglycerate.
  • Authors: Kurup PA, Arun P, Gayathri NS, Dhanya CR, Indu AR
  • Issue date: 2003 Nov
  • Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1.
  • Authors: Mittag D, Sran A, Chan KS, Boland MP, Bandala-Sanchez E, Huet O, Xu W, Sparrow RL
  • Issue date: 2015 Sep
  • In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study.
  • Authors: Sparrow RL, Sran A, Healey G, Veale MF, Norris PJ
  • Issue date: 2014 Mar

DSpace software (copyright © 2002 - 2022)  DuraSpace
Quick Guide | Contact Us
Open Repository is a service operated by 
Atmire NV
 

Export search results

The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.