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dc.contributor.authorPaglia, Giuseppe
dc.contributor.authorSigurjónsson, Ólafur E
dc.contributor.authorBordbar, Aarash
dc.contributor.authorRolfsson, Óttar
dc.contributor.authorMagnusdottir, Manuela
dc.contributor.authorPalsson, Sirus
dc.contributor.authorWichuk, Kristine
dc.contributor.authorGudmundsson, Sveinn
dc.contributor.authorPalsson, Bernhard O
dc.date.accessioned2019-12-13T14:38:11Z
dc.date.available2019-12-13T14:38:11Z
dc.date.issued2016-10
dc.date.submitted2019-12
dc.identifier.citationPaglia G, Sigurjónsson ÓE, Bordbar A, Rolfsson Ó, Magnusdottir M, Palsson S, Wichuk K, Gudmundsson S, Palsson BO. Metabolic fate of adenine in red blood cells during storage in SAGM solution. Transfusion. 2016 Oct;56(10):2538-47.en_US
dc.identifier.pmid27491795
dc.identifier.doi10.1111/trf.13740
dc.identifier.urihttp://hdl.handle.net/2336/621230
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen_US
dc.description.abstractBACKGROUND: Red blood cells (RBCs) are routinely stored and transfused worldwide. Recently, metabolomics have shown that RBCs experience a three-phase metabolic decay process during storage, resulting in the definition of three distinct metabolic phenotypes, occurring between Days 1 and 10, 11 and 17, and 18 and 46. Here we use metabolomics and stable isotope labeling analysis to study adenine metabolism in RBCs. STUDY DESIGN AND METHODS: A total of 6 units were prepared in SAGM or modified additive solutions (ASs) containing 15 N5 -adenine. Three of them were spiked with 15 N5 -adenine on Days 10, 14, and 17 during storage. Each unit was sampled 10 times spanning Day 1 to Day 32. At each time point metabolic profiling was performed. RESULTS: We increased adenine concentration in the AS and we pulsed the adenine concentration during storage and found that in both cases the RBCs' main metabolic pathways were not affected. Our data clearly show that RBCs cannot consume adenine after 18 days of storage, even if it is still present in the storage solution. However, increased levels of adenine influenced S-adenosylmethionine metabolism. CONCLUSION: In this work, we have studied in detail the metabolic fate of adenine during RBC storage in SAGM. Adenine is one of the main substrates used by RBCs, but the metabolic shift observed during storage is not caused by an absence of adenine later in storage. The rate of adenine consumption strongly correlated with duration of storage but not with the amount of adenine present in the AS.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/trf.13740en_US
dc.relation.urlhttps://ovidsp.dc1.ovid.com/sp-4.04.0a/ovidweb.cgi?QS2=434f4e1a73d37e8c740ce3cde7a337850ed3a6d9c4b79c7d5cd5f3b623205df04f7bbe199b95c9a83c599c8f9ba730aa272a1f298e37b58bf87e10f373191fd16548e47eaa843979f083c232cabb05d99e5616c83e54f8a6845c35cb32138809cd65c326f5954c7ec5f7d8489692c5a17e1434bbb124968bda48edee9349572855336d44014ed972df405f515450412b85744adf893493c51be3580b91740e35772b574274e2b381ffb78b7cb6143468f7f2142b90537b9e441fa7193932c50a4c319b424f833309b456ddf8270e55874b13bdf1b81fdcd90560e31a1743988944009a3b6c3f034a1a4a88d73d25777332867df94390f83621d2ced5178cbc67ce8628fa2ba84b3d3005748822991c637796cc97df29c1e8907c4af54b9f40cdf008ada778fd54e6a08c3e8c73ec28d87707e7180b0e20d9cd8793bd16810d2fe657893dde1914d171badbcfb469924e31c2740db25aaa3186597a72b57787ea7669cc6fb001ba2a2196628d0ce14e267ed0d01fed97d8759d48a9cf69e3685c2a87c73030e56256659dc5e07d69bbfed74ca7a357cfec630e84921d28c80cfe97943a93c355c97a18c57f97ecc0f475966a56e0d1657ba3dccefd006f633987549e71be9fb830b52411aaedb27ceb6b4c17a81cfec911f5bda2d2ddbbc1bbeden_US
dc.rights© 2016 AABB.
dc.subjectBlóðkornen_US
dc.subjectBlóðbankaren_US
dc.subject.meshAdenineen_US
dc.subject.meshErythrocytesen_US
dc.subject.meshBlood Preservationen_US
dc.titleMetabolic fate of adenine in red blood cells during storage in SAGM solution.en_US
dc.typeArticleen_US
dc.identifier.eissn1537-2995
dc.contributor.department1 Center for Biomedicine, European Academy of Bolzano/Bozen, Bolzano, Italy. giuseppe.paglia@eurac.edu. 2 Center for Systems Biology, University of Iceland, Reykjavik, Iceland;. giuseppe.paglia@eurac.edu. 3 The Blood Bank, Landspitali-University Hospital, Reykjavik, Iceland. 4 School of Science and Engineering, Reykjavik University, Reykjavik, Iceland; and. 5 Sinopia Biosciences, San Diego, California. 6 Center for Systems Biology, University of Iceland, Reykjavik, Iceland.en_US
dc.identifier.journalTransfusionen_US
dc.rights.accessNational Consortium - Landsaðganguren_US
dc.source.journaltitleTransfusion
dc.source.volume56
dc.source.issue10
dc.source.beginpage2538
dc.source.endpage2547
dc.source.countryUnited States


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