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dc.contributor.authorMorera, Erika
dc.contributor.authorSteinhäuser, Sarah Sophie
dc.contributor.authorBudkova, Zuzana
dc.contributor.authorIngthorsson, Saevar
dc.contributor.authorKricker, Jennifer
dc.contributor.authorKrueger, Aileen
dc.contributor.authorTraustadottir, Gunnhildur Asta
dc.contributor.authorGudjonsson, Thorarinn
dc.date.accessioned2019-12-23T09:28:54Z
dc.date.available2019-12-23T09:28:54Z
dc.date.issued2019-12
dc.date.submitted2019-12
dc.identifier.citationMorera E, Steinhäuser SS, Budkova Z, Ingthorsson S, Kricker J, Krueger A, Traustadottir GA, Gudjonsson T. YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation. In Vitro Cell Dev Biol Anim. 2019 Dec;55(10):838-853. doi: 10.1007/s11626-019-00403-x.en_US
dc.identifier.pmid31482369
dc.identifier.doi10.1007/s11626-019-00403-x
dc.identifier.urihttp://hdl.handle.net/2336/621252
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Downloaden_US
dc.description.abstractEpithelial to mesenchymal transition (EMT) is a developmental event that is hijacked in some diseases such as fibrosis and cancer. In cancer, EMT has been linked to increased invasion and metastasis and is generally associated with a poor prognosis. In this study, we have compared phenotypic and functional differences between two isogenic cell lines with an EMT profile: D492M and D492HER2 that are both derived from D492, a breast epithelial cell line with stem cell properties. D492M is non-tumorigenic while D492HER2 is tumorigenic. Thus, the aim of this study was to analyze the expression profile of these cell lines, identify potential oncogenes, and evaluate their effects on cellular phenotype. We performed transcriptome and secretome analyses of D492M and D492HER2 and verified expression of selected genes at the RNA and protein level. One candidate, YKL-40 (also known as CHI3L1), was selected for further studies due to its differential expression between D492M and D492HER2, being considerably higher in D492HER2. YKL-40 has been linked to chronic inflammation diseases and cancer, yet its function is not fully understood. Knock-down experiments of YKL-40 in D492HER2 resulted in reduced migration and invasion as well as reduced ability to induce angiogenesis in an in vitro assay, plus changes in the EMT-phenotype. In summary, our data suggest that YKL-40 may provide D492HER2 with increased aggressiveness, supporting cancer progression and facilitating angiogenesis.en_US
dc.description.sponsorshipUniversity of Iceland Research fund Landspitali Haskolasjukrahus Science fund Gongum saman supporting group for breast cancer research in Iceland Grant of Excellence, Icelandic Science and Technology Policyen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs11626-019-00403-xen_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881255/en_US
dc.subjectAngiogenesisen_US
dc.subjectEpithelial to mesenchymal transition (EMT)en_US
dc.subjectInvasive breast canceren_US
dc.subjectMigrationen_US
dc.subjectYKL-40/CHI3L1en_US
dc.subjectBrjóstakrabbamein
dc.subject.meshNeoplastic Stem Cellsen_US
dc.titleYKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation.en_US
dc.typeArticleen_US
dc.identifier.eissn1543-706X
dc.contributor.department1 Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Vatnsmyrarvegi 16, 101, Reykjavik, Iceland. 2 Heidelberg Institute for Stem Cell Technology and Experimental Medicine, Heidelberg, Germany. 3 Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Vatnsmyrarvegi 16, 101, Reykjavik, Iceland. tgudjons@hi.is. 4 Department of Laboratory Hematology, Landspitali - University Hospital, Reykjavik, Iceland. tgudjons@hi.is.en_US
dc.identifier.journalIn vitro cellular & developmental biology. Animalen_US
dc.rights.accessOpen Access - Opinn aðganguren_US
dc.departmentcodeNAF12
dc.source.journaltitleIn vitro cellular & developmental biology. Animal
dc.source.volume55
dc.source.issue10
dc.source.beginpage838
dc.source.endpage853
refterms.dateFOA2019-12-23T09:28:55Z
dc.source.countryGermany


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