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dc.contributor.authorJóhannsson, Freyr
dc.contributor.authorÁrnason, Níels Á
dc.contributor.authorLandrö, Ragna
dc.contributor.authorGuðmundsson, Sveinn
dc.contributor.authorSigurjonsson, Ólafur E
dc.contributor.authorRolfsson, Óttar
dc.date.accessioned2020-01-02T12:46:06Z
dc.date.available2020-01-02T12:46:06Z
dc.date.issued2019-12-05
dc.date.submitted2020-01
dc.identifier.citationJóhannsson F, Árnason NÁ, Landrö R, Guðmundsson S, Sigurjonsson ÓE, Rolfsson Ó. Metabolomics study of platelet concentrates photochemically treated with amotosalen and UVA light for pathogen inactivation. Transfusion. 2020;60(2):367‐377. doi:10.1111/trf.15610en_US
dc.identifier.pmid31802514
dc.identifier.doi10.1111/trf.15610
dc.identifier.urihttp://hdl.handle.net/2336/621256
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen_US
dc.description.abstractBACKGROUND: The risk of bacterial contamination and the deterioration of platelet (PLT) quality limit the shelf-life of platelet concentrates (PCs). The INTERCEPT pathogen inactivation system reduces the risk of pathogen transmission by inhibiting nucleic acid replication using a combination of a photo-reactive compound and UVA illumination. The goal of this study was to investigate the effects the INTERCEPT system has on the PLT metabolome and metabolic activity. STUDY DESIGN AND METHODS: Paired units of buffy coat-derived PCs were generated using a pool and split strategy (n = 8). The paired PCs were either treated with the INTERCEPT system or left untreated. Samples were collected on Days 1, 2, 4, and 7 of storage. Ultra-performance chromatography coupled with time-of-flight mass spectrometry was used to analyze the extra- and intracellular metabolomes. Constraint-based metabolic modeling was then used to predict the metabolic activity of the stored PLTs. RESULTS: A relatively large number of metabolites in the extracellular environment were depleted during the processing steps of the INTERCEPT system, in particular, metabolites with hydrophobic functional groups, including acylcarnitines and lysophosphatidylcholines. In the intracellular environment, alterations in glucose and glycerophospholipid metabolism and decreased levels of 2-hydroxyglutarate were observed following the INTERCEPT treatment. Untargeted metabolomics analysis revealed residual amotosalen dimers present in the treated PCs. Systems-level analysis of PLT metabolism indicated that the INTERCEPT system does not have a significant impact on the PLT energy metabolism and nutrient utilization. CONCLUSIONS: The INTERCEPT system significantly alters the metabolome of the stored PCs without significantly influencing PLT energy metabolism.en_US
dc.description.sponsorshipLandspitali University Hospital Research Fund University of Icelanden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/trf.15610en_US
dc.rights© 2019 AABB.
dc.subjectBlóðflöguren_US
dc.subject.meshBlood Plateletsen_US
dc.titleMetabolomics study of platelet concentrates photochemically treated with amotosalen and UVA light for pathogen inactivation.en_US
dc.typeArticleen_US
dc.identifier.eissn1537-2995
dc.contributor.department1 Center for Systems Biology, University of Iceland, Sturlugata 8, Reykjavik, Iceland. 2 Medical Department, University of Iceland, Sturlugata 8, Reykjavik, Iceland. 3 The Blood Bank, Landspitali-University Hospital, Snorrabraut 60, Reykjavik, Iceland. 4 School of Science and Engineering, Reykjavik University, Menntavegur 1, Reykjavik, Iceland.en_US
dc.identifier.journalTransfusionen_US
dc.rights.accessNational Consortium - Landsaðganguren_US
dc.departmentcodeNAF12
dc.departmentcodeBAB12
dc.source.journaltitleTransfusion
dc.source.countryUnited States


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