Endothelin-1 increases expression and activity of arginase 2 via ETB receptors and is co-expressed with arginase 2 in human atherosclerotic plaques.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Matic, Ljubica Perisic
Hansson, Göran K
MetadataShow full item record
CitationRafnsson A, Matic LP, Lengquist M, et al. Endothelin-1 increases expression and activity of arginase 2 via ETB receptors and is co-expressed with arginase 2 in human atherosclerotic plaques. Atherosclerosis. 2020;292:215–223. doi:10.1016/j.atherosclerosis.2019.09.020
AbstractBackground and aims: Endothelin-1 (ET-1) and arginase are both suggested to be involved in the inflammatory processes and development of endothelial dysfunction in atherosclerosis. However, information regarding the roles of ET-1 and arginase, as well as the interactions between the two in human atherosclerosis, is scarce. We investigated the expression of ET-1 and its receptors, ETA and ETB, as well as arginase in human carotid atherosclerotic plaques and determined the functional interactions between ET-1 and arginase in endothelial cells and THP-1-derived macrophages. Methods: Carotid plaques and blood samples were retreived from patients undergoing surgery for symptomatic or asymptomatic carotid stenosis. Plaque gene and protein expression was determined and related to clinical characteristics. Functional interactions between ET-1 and arginase were investigated in endothelial cells and THP-1 cells. Results: Expression of ET-1 and ETB receptors was increased in plaques from patients with symptomatic carotid artery disease. ET-1 was co-localized with arginase 1 and arginase 2 in the necrotic core, together with macrophage markers CD163 and CD68. Arginase 2, ET-1 and ETB receptors were expressed in endothelial cells as well as in smooth muscle cells in the fibrous cap. ET-1 increased arginase 2 mRNA expression and arginase activity in endothelial cells and arginase activity in macrophages. Moreover, ET-1 stimulated formation of reactive oxygen species (ROS) in THP-1-derived macrophages via an arginase-dependent mechanism. Conclusions: This is the first study that demonstrates co-localization of ET-1 and arginase 2 in human atherosclerotic plaques. ET-1 stimulated arginase 2 expression and activity in endothelial cells, as well as arginase activity and ROS formation in macrophages via an arginase-dependent mechanism. These results indicate an important interaction between the ET pathway and arginase in human atherosclerotic plaques.
DescriptionTo access publisher's full text version of this article click on the hyperlink below
RightsCopyright © 2019 Elsevier B.V. All rights reserved.
- Functional characterization and expression of endothelin receptors in rat carotid artery: involvement of nitric oxide, a vasodilator prostanoid and the opening of K+ channels in ETB-induced relaxation.
- Authors: Tirapelli CR, Casolari DA, Yogi A, Montezano AC, Tostes RC, Legros E, D'Orléans-Juste P, de Oliveira AM
- Issue date: 2005 Nov
- Role of endothelin receptors on basal and endothelin-1-stimulated lung myofibroblast proliferation.
- Authors: Préfontaine A, Calderone A, Dupuis J
- Issue date: 2008 Jun
- Endothelin-1 and ETA/ETB receptor protein and mRNA: expression in normal and vascularized human corneas.
- Authors: Kuhlmann A, Amann K, Schlötzer-Schrehardt U, Kruse FE, Cursiefen C
- Issue date: 2005 Oct
- Non-endothelial cell endothelin-B receptors limit neointima formation following vascular injury.
- Authors: Kirkby NS, Duthie KM, Miller E, Kotelevtsev YV, Bagnall AJ, Webb DJ, Hadoke PW
- Issue date: 2012 Jul 1
- PKC-Mediated Endothelin-1 Expression in Endothelial Cell Promotes Macrophage Activation in Atherogenesis.
- Authors: Zhang J, Wang YJ, Wang X, Xu L, Yang XC, Zhao WS
- Issue date: 2019 Aug 14