Reduced Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the Gene Is Geographically Widespread.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Musser, James MBeres, Stephen B
Zhu, Luchang
Olsen, Randall J
Vuopio, Jaana
Hyyryläinen, Hanne-Leena
Gröndahl-Yli-Hannuksela, Kirsi
Kristinsson, Karl G
Darenberg, Jessica
Henriques-Normark, Birgitta
Hoffmann, Steen
Caugant, Dominque A
Smith, Andrew J
Lindsay, Diane S J
Boragine, David M
Palzkill, Timothy
Issue Date
2020-03-25
Metadata
Show full item recordCitation
Musser JM, Beres SB, Zhu L, et al. Reduced In Vitro Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread. J Clin Microbiol. 2020;58(4):e01993-19. Published 2020 Mar 25. doi:10.1128/JCM.01993-19Abstract
Recently, two related Streptococcus pyogenes strains with reduced susceptibility to ampicillin, amoxicillin, and cefotaxime, antibiotics commonly used to treat S. pyogenes infections, were reported. The two strains had the same nonsynonymous (amino acid-substituting) mutation in the pbp2x gene, encoding penicillin-binding protein 2X (PBP2X). This concerning report led us to investigate our library of 7,025 genome sequences of type emm1, emm28, and emm89S. pyogenes clinical strains recovered from intercontinental sources for mutations in pbp2x We identified 137 strains that, combined, had 37 nonsynonymous mutations in 36 codons in pbp2x Although to a lesser magnitude than the two previously published isolates, many of our strains had decreased susceptibility in vitro to multiple beta-lactam antibiotics. Many pbp2x mutations were found only in single strains, but 16 groups of two or more isolates of the same emm type had an identical amino acid replacement. Phylogenetic analysis showed that, with one exception, strains of the same emm type with the same amino acid replacement were clonally related by descent. This finding indicates that strains with some amino acid changes in PBP2X can successfully spread to new human hosts and cause invasive infections. Mapping of the amino acid changes onto a three-dimensional structure of the related Streptococcus pneumoniae PBP2X suggests that some substitutions are located in regions functionally important in related pathogenic bacterial species. Decreased beta-lactam susceptibility is geographically widespread in strains of numerically common emm gene subtypes. Enhanced surveillance and further epidemiological and molecular genetic study of this potential emergent antimicrobial problem are warranted.Description
To access publisher's full text version of this article click on the hyperlink belowAdditional Links
https://jcm.asm.org/content/58/4/e01993-19https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098749/
Rights
Copyright © 2020 American Society for Microbiology.ae974a485f413a2113503eed53cd6c53
10.1128/JCM.01993-19
Scopus Count
Collections
Related articles
- Restricted Sequence Variation in Streptococcus pyogenes Penicillin Binding Proteins.
- Authors: Hayes A, Lacey JA, Morris JM, Davies MR, Tong SYC
- Issue date: 2020 Apr 29
- Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506.
- Authors: Laible G, Hakenbeck R, Sicard MA, Joris B, Ghuysen JM
- Issue date: 1989 Oct
- [Effects of amino acid substitutions of penicillin-binding proteins 2B, 1A, 2X on minimal inhibitory concentration of beta-lactams against Streptococcus pneumoniae].
- Authors: Xu M, Zhang JH, Ding YF, Tao YZ, Wang ZC
- Issue date: 2010 Jan
- [Evaluation of penicillin-binding protein genotypes in penicillin susceptible and resistant Streptococcus pneumoniae isolates].
- Authors: Aslan G, Tezcan S, Delialioğlu N, Aydın FE, Kuyucu N, Emekdaş G
- Issue date: 2012 Apr
- Streptococcus pyogenes pbp2x Mutation Confers Reduced Susceptibility to β-Lactam Antibiotics.
- Authors: Vannice KS, Ricaldi J, Nanduri S, Fang FC, Lynch JB, Bryson-Cahn C, Wright T, Duchin J, Kay M, Chochua S, Van Beneden CA, Beall B
- Issue date: 2020 Jun 24