Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation.
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Authors
Kurek, MagdalenaÅkesson, Elisabet
Yoshihara, Masahito
Oliver, Elizabeth
Cui, Yanhua
Becker, Martin
Alves-Lopes, João Pedro
Bjarnason, Ragnar
Romerius, Patrik
Sundin, Mikael
Norén Nyström, Ulrika
Langenskiöld, Cecilia
Vogt, Hartmut
Henningsohn, Lars
Petersen, Cecilia
Söder, Olle
Guo, Jingtao
Mitchell, Rod T
Jahnukainen, Kirsi
Stukenborg, Jan-Bernd
Issue Date
2021-01-27
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Kurek M, Åkesson E, Yoshihara M, Oliver E, Cui Y, Becker M, Alves-Lopes JP, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Petersen C, Söder O, Guo J, Mitchell RT, Jahnukainen K, Stukenborg JB. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells. 2021 Jan 27;10(2):241. doi: 10.3390/cells10020241.Abstract
Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. Keywords: Sertoli cells; basal membrane; germ cells; infertility; late effects; seminiferous tubules; spermatogonia; stem cell niche.Description
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https://www.mdpi.com/2073-4409/10/2/241https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/
ae974a485f413a2113503eed53cd6c53
10.3390/cells10020241
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