Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Alves-Lopes, João Pedro
Norén Nyström, Ulrika
Mitchell, Rod T
MetadataShow full item record
CitationKurek M, Åkesson E, Yoshihara M, Oliver E, Cui Y, Becker M, Alves-Lopes JP, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Petersen C, Söder O, Guo J, Mitchell RT, Jahnukainen K, Stukenborg JB. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells. 2021 Jan 27;10(2):241. doi: 10.3390/cells10020241.
AbstractFertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. Keywords: Sertoli cells; basal membrane; germ cells; infertility; late effects; seminiferous tubules; spermatogonia; stem cell niche.
DescriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download
- Irradiation affects germ and somatic cells in prepubertal monkey testis xenografts.
- Authors: Tröndle I, Westernströer B, Wistuba J, Terwort N, Schlatt S, Neuhaus N
- Issue date: 2017 Mar 1
- Preserved seminiferous tubule integrity with spermatogonial survival and induction of Sertoli and Leydig cell maturation after long-term organotypic culture of prepubertal human testicular tissue.
- Authors: de Michele F, Poels J, Weerens L, Petit C, Evrard Z, Ambroise J, Gruson D, Wyns C
- Issue date: 2017 Jan
- In vitro differentiation of rat spermatogonia into round spermatids in tissue culture.
- Authors: Reda A, Hou M, Winton TR, Chapin RE, Söder O, Stukenborg JB
- Issue date: 2016 Sep
- Evaluation of apoptotic- and autophagic-related protein expressions before and after IVM of fresh, slow-frozen and vitrified pre-pubertal mouse testicular tissue.
- Authors: Dumont L, Chalmel F, Oblette A, Berby B, Rives A, Duchesne V, Rondanino C, Rives N
- Issue date: 2017 Nov 1
- Vitrified canine testicular cells allow the formation of spermatogonial stem cells and seminiferous tubules following their xenotransplantation into nude mice.
- Authors: Lee KH, Lee WY, Kim DH, Lee SH, Do JT, Park C, Kim JH, Choi YS, Song H
- Issue date: 2016 Feb 24