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dc.contributor.authorKurek, Magdalena
dc.contributor.authorÅkesson, Elisabet
dc.contributor.authorYoshihara, Masahito
dc.contributor.authorOliver, Elizabeth
dc.contributor.authorCui, Yanhua
dc.contributor.authorBecker, Martin
dc.contributor.authorAlves-Lopes, João Pedro
dc.contributor.authorBjarnason, Ragnar
dc.contributor.authorRomerius, Patrik
dc.contributor.authorSundin, Mikael
dc.contributor.authorNorén Nyström, Ulrika
dc.contributor.authorLangenskiöld, Cecilia
dc.contributor.authorVogt, Hartmut
dc.contributor.authorHenningsohn, Lars
dc.contributor.authorPetersen, Cecilia
dc.contributor.authorSöder, Olle
dc.contributor.authorGuo, Jingtao
dc.contributor.authorMitchell, Rod T
dc.contributor.authorJahnukainen, Kirsi
dc.contributor.authorStukenborg, Jan-Bernd
dc.date.accessioned2021-04-20T13:59:13Z
dc.date.available2021-04-20T13:59:13Z
dc.date.issued2021-01-27
dc.date.submitted2021-04
dc.identifier.citationKurek M, Åkesson E, Yoshihara M, Oliver E, Cui Y, Becker M, Alves-Lopes JP, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Petersen C, Söder O, Guo J, Mitchell RT, Jahnukainen K, Stukenborg JB. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells. 2021 Jan 27;10(2):241. doi: 10.3390/cells10020241.en_US
dc.identifier.pmid33513766
dc.identifier.doi10.3390/cells10020241
dc.identifier.urihttp://hdl.handle.net/2336/621753
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Downloaden_US
dc.description.abstractFertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. Keywords: Sertoli cells; basal membrane; germ cells; infertility; late effects; seminiferous tubules; spermatogonia; stem cell niche.en_US
dc.description.sponsorshipJane and Dan Olssons Foundation Frimurare Barnhuset i Stockholm Kronprinsessan Lovisas Forening For Barnasjukvard/Stiftelsen Axel Tielmans Minnesfond Samariten Foundation Tore Nilsson Foundation European Commission Magnus Bergvalls Stiftelsen Swedish Research Council UKRI Future Leaders Fellowship UK Research & Innovation (UKRI) Medical Research Council UK (MRC) ITN Marie Curie program "Growsperm" Karolinska Institutet Scandinavia-Japan Sasakawa Foundation Japan Eye Bank Association Astellas Foundation for Research on Metabolic Disorders Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Birgitta and Carl-Axel Rydbeck's Research Grant for Pediatric Researchen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.mdpi.com/2073-4409/10/2/241en_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/en_US
dc.subjectSertoli cellsen_US
dc.subjectbasal membraneen_US
dc.subjectgerm cellsen_US
dc.subjectinfertilityen_US
dc.subjectlate effectsen_US
dc.subjectseminiferous tubulesen_US
dc.subjectspermatogoniaen_US
dc.subjectstem cell nicheen_US
dc.subjectFrjósemien_US
dc.subjectKrabbameinen_US
dc.subjectDrengiren_US
dc.titleSpermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation.en_US
dc.typeArticleen_US
dc.identifier.eissn2073-4409
dc.contributor.department1NORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, Sweden. 2Division of Neurogeriatrics, Department of Neurobiology Care Sciences & Society, Karolinska Institutet, 141 83 Huddinge, Sweden. 3The R & D Unit, Stockholms Sjukhem, 112 19 Stockholm, Sweden. 4Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden. 5Center of Neurodevelopmental Disorders (KIND), Department of Women's and Children's Health, Karolinska Institutet, Centre for Psychiatry Research, Region Stockholm and Astrid Lindgren Children's Hospital, Karolinska University Hospital, 171 64 Solna, Sweden. 6Children's Medical Center, Landspítali University Hospital, 101 Reykjavik, Iceland. 7Department of Paediatrics Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland. 8Department of Paediatric Oncology and Haematology, Clinical Sciences, Lund University, Barn-och Ungdomssjukhuset Lund, Skånes Universitetssjukhus, 221 85 Lund, Sweden. 9Division of Paediatrics, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, Sweden. 10Pediatric Blood Disorders, Immunodeficiency and Stem Cell Transplantation Unit, Astrid Lindgren Children's Hospital, Karolinska University Hospital, 141 86 Huddinge, Sweden. 11Division of Paediatrics, Department of Clinical Science, Umeå University, 901 87 Umeå, Sweden. 12Department of Paediatric Oncology, The Queen Silvia Children's Hospital, 416 50 Gothenburg, Sweden. 13Crown Princess Victoria's Child and Youth Hospital, and Department of Biomedical and Clinical Sciences, Linköping University, 581 83 Linköping, Sweden. 14Division of Urology, Institution for Clinical Science Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, Sweden. 15Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84112, USA. 16MRC Centre for Reproductive Health, The Queen's Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UK. 17Edinburgh Royal Hospital for Sick Children, Edinburgh EH9 1LF, UK. 18Division of Haematology-Oncology and Stem Cell Transplantation, Children's Hospital, University of Helsinki, Helsinki University Central Hospital, 00029 Helsinki, Finland.en_US
dc.identifier.journalCellsen_US
dc.rights.accessOpen Access - Opinn aðganguren_US
dc.departmentcodePED12
dc.source.journaltitleCells
dc.source.volume10
dc.source.issue2
refterms.dateFOA2021-04-20T13:59:13Z
dc.source.countryUnited Kingdom
dc.source.countryUnited Kingdom
dc.source.countrySwitzerland


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