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dc.contributor.authorMilewska, Adrianna
dc.contributor.authorSigurjonsson, Olafur E.
dc.contributor.authorLeosson, Kristjan
dc.date.accessioned2021-11-03T14:08:02Z
dc.date.available2021-11-03T14:08:02Z
dc.date.issued2021-06-21
dc.date.submitted2021-11
dc.identifier.citationMilewska A, Sigurjonsson OE, Leosson K. SERS Imaging of Mesenchymal Stromal Cell Differentiation. ACS Applied Bio Materials. 2021;4(6):4999-5007.doi:10.1021/acsabm.1c00286.en_US
dc.identifier.doi10.1021/acsabm.1c00286
dc.identifier.urihttp://hdl.handle.net/2336/621937
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen_US
dc.description.abstractUnderstanding the process of mesenchymal stromal cell (MSC) osteogenic differentiation is essential for a wide range of medical applications. However, these primary cells vary significantly from donor to donor, making it difficult to fully exploit their therapeutic potential. Although osteogenic differentiation has been studied extensively, there is still a shortage of standardized methods for the evaluation of the degree of differentiation. Here, we employ noninvasive surface-enhanced Raman scattering (SERS) for studying such cells, offering a better understanding of cellular processes in situ. We present the long-term differentiation of MSCs on biocompatible gold nanoisland SERS substrates, combining imaging of cells with spectroscopic detection of molecular species and chemical events occurring on the cellular membrane adjacent to the surface of the SERS substrate. We detect multiple signs of bone tissue formation, from an early stage to mature osteoblasts, without labeling. We show that the results correlate very well with classical differentiation-detecting assays, indicating that the SERS imaging technique alone is sufficient to study the progress of osteogenic differentiation of such cells, paving a way toward continuous label-free screening of live cells. © 2021 American Chemical Society. Author keywords extracellular matrix; hydroxyapatite; mesenchymal stromal cells; osteogenic differentiation; SERS substrates; stem cells; surface-enhanced Raman scattering (SERS)en_US
dc.description.sponsorshipUniversity of Iceland Research Fund Landspitali University Hospital Research funden_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acsabm.1c00286en_US
dc.subjectextracellular matrixen_US
dc.subjecthydroxyapatiteen_US
dc.subjectmesenchymal stromal cellsen_US
dc.subjectosteogenic differentiationen_US
dc.subjectSERS substratesen_US
dc.subjectstem cellsen_US
dc.subjectsurface-enhanced Raman scattering (SERS)en_US
dc.titleSERS Imaging of Mesenchymal Stromal Cell Differentiationen_US
dc.typeArticleen_US
dc.identifier.eissn25766422
dc.contributor.departmenta Innovation Center Iceland, árleynir 2-8, Reykjavík, 112, Iceland b The Blood Bank, Landspitali University Hospital, Snorrabraut 60, Reykjavík, 105, Iceland c University of Iceland, School of Engineering and Natural Sciences, Sæmundargötu 2, Reykjavík, 101, Iceland d Reykjavik University, School of Science and Engineering, Menntavegur 1, Reykjavík, 101, Icelanden_US
dc.identifier.journalACS Applied Bio Materialsen_US
dc.rights.accessClosed - Lokaðen_US
dc.departmentcodeNAF12
dc.identifier.eid2-s2.0-85108518988
dc.identifier.scopusidSCOPUS_ID:85108518988
dc.source.journaltitleACS Applied Bio Materials
dc.source.volume4
dc.source.issue6
dc.source.beginpage4999
dc.source.endpage5007


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