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dc.contributor.authorSigmarsdottir, Thora Bjorg
dc.contributor.authorMcGarrity, Sarah
dc.contributor.authorYurkovich, James T
dc.contributor.authorRolfsson, Óttar
dc.contributor.authorSigurjónsson, Ólafur Eysteinn
dc.date.accessioned2021-11-10T14:58:39Z
dc.date.available2021-11-10T14:58:39Z
dc.date.issued2021-06-04
dc.date.submitted2021-11
dc.identifier.citationSigmarsdottir TB, McGarrity S, Yurkovich JT, Rolfsson Ó, Sigurjónsson ÓE. Analyzing Metabolic States of Adipogenic and Osteogenic Differentiation in Human Mesenchymal Stem Cells via Genome Scale Metabolic Model Reconstruction. Front Cell Dev Biol. 2021;9:642681. Published 2021 Jun 4. doi:10.3389/fcell.2021.642681en_US
dc.identifier.issn2296-634X
dc.identifier.pmid34150750
dc.identifier.doi10.3389/fcell.2021.642681
dc.identifier.urihttp://hdl.handle.net/2336/621952
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Downloaden_US
dc.description.abstractSince their initial discovery in 1976, mesenchymal stem cells (MSCs) have been gathering interest as a possible tool to further the development and enhancement of various therapeutics within regenerative medicine. However, our current understanding of both metabolic function and existing differences within the varying cell lineages (e.g., cells in either osteogenesis or adipogenesis) is severely lacking making it more difficult to fully realize the therapeutic potential of MSCs. Here, we reconstruct the MSC metabolic network to understand the activity of various metabolic pathways and compare their usage under different conditions and use these models to perform experimental design. We present three new genome-scale metabolic models (GEMs) each representing a different MSC lineage (proliferation, osteogenesis, and adipogenesis) that are biologically feasible and have distinctive cell lineage characteristics that can be used to explore metabolic function and increase our understanding of these phenotypes. We present the most distinctive differences between these lineages when it comes to enriched metabolic subsystems and propose a possible osteogenic enhancer. Taken together, we hope these mechanistic models will aid in the understanding and therapeutic potential of MSCs. Keywords: GEM; MSCs; adipogenesis; metabolic differences; metabolic reconstruction; osteogenesis.en_US
dc.description.sponsorshipIcelandic Research Funden_US
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fcell.2021.642681/fullen_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212021/en_US
dc.rightsCopyright © 2021 Sigmarsdottir, McGarrity, Yurkovich, Rolfsson and Sigurjónsson.
dc.subjectGEMen_US
dc.subjectMSCsen_US
dc.subjectadipogenesisen_US
dc.subjectmetabolic differencesen_US
dc.subjectmetabolic reconstructionen_US
dc.subjectosteogenesisen_US
dc.titleAnalyzing Metabolic States of Adipogenic and Osteogenic Differentiation in Human Mesenchymal Stem Cells via Genome Scale Metabolic Model Reconstruction.en_US
dc.typeArticleen_US
dc.contributor.department1School of Science and Engineering, Reykjavík University, Reykjavík, Iceland. 2Center for Systems Biology, University of Iceland, Reykjavík, Iceland. 3Department of Bioengineering, University of California, San Diego, La Jolla, CA, United States. 4The Blood Bank, Landspitali - The National University Hospital of Iceland, Reykjavík, Iceland.en_US
dc.identifier.journalFrontiers in cell and developmental biologyen_US
dc.rights.accessOpen Access - Opinn aðganguren_US
dc.departmentcodeBAB12
dc.departmentcodeNAF12
dc.source.journaltitleFrontiers in cell and developmental biology
dc.source.volume9
dc.source.beginpage642681
dc.source.endpage
refterms.dateFOA2021-11-10T14:58:39Z
dc.source.countrySwitzerland


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