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dc.contributor.authorŽivanović, Vesna
dc.contributor.authorMilewska, Adrianna
dc.contributor.authorLeosson, Kristjan
dc.contributor.authorKneipp, Janina
dc.date.accessioned2021-11-30T15:52:21Z
dc.date.available2021-11-30T15:52:21Z
dc.date.issued2021-07-15
dc.date.submitted2021-11
dc.identifier.citationŽivanović V, Milewska A, Leosson K, Kneipp J. Molecular Structure and Interactions of Lipids in the Outer Membrane of Living Cells Based on Surface-Enhanced Raman Scattering and Liposome Models. Anal Chem. 2021;93(29):10106-10113. doi:10.1021/acs.analchem.1c00964en_US
dc.identifier.pmid34264630
dc.identifier.doi10.1021/acs.analchem.1c00964
dc.identifier.urihttp://hdl.handle.net/2336/621966
dc.descriptionTo access publisher's full text version of this article click on the hyperlink belowen_US
dc.description.abstractThe distribution and interaction of lipids determine the structure and function of the cellular membrane. Surface-enhanced Raman scattering (SERS) is used for selective molecular probing of the cell membrane of living fibroblast cells grown adherently on gold nanoisland substrates across their whole contact areas with the substrate, enabling mapping of the membrane's composition and interaction. From the SERS data, the localization and distribution of different lipids and their interactions, together with proteins in the outer cell membrane, are inferred. Interpretation of the spectra is mainly supported by comparison with the spectra of model liposomes composed of phosphatidylcholine, sphingomyelin, and cholesterol obtained on the same gold substrate. The interaction of the liposomes with the substrate differs from that with gold nanoparticles. The SERS maps indicate colocalization of ordered lipid domains with cholesterol in the living cells. They support the observation of ordered membrane regions of micrometer dimensions in the outer leaflet of the cell membrane that are rich in sphingomyelin. Moreover, the spectra of the living cells contain bands from the groups of the lipid heads, phosphate, choline, and ethanolamine, combined with those from membrane proteins, as indicated by signals assigned to prenyl attachment. Elucidating the composition and structure of lipid membranes in living cells can find application in many fields of research.en_US
dc.description.sponsorshipEuropean Research Council (ERC) European Commission Icelandic Research Fund University of Iceland Research Funden_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acs.analchem.1c00964#en_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshLiposomesen_US
dc.titleMolecular Structure and Interactions of Lipids in the Outer Membrane of Living Cells Based on Surface-Enhanced Raman Scattering and Liposome Models.en_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.identifier.eissn1520-6882
dc.contributor.department1Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, Berlin 12489, Germany. 2Innovation Center Iceland, Árleynir 2-8, Reykjavík 112, Iceland. 3The Blood Bank, Landspitali University Hospital, Snorrabraut 60, Reykjavík 105, Iceland. 4School of Engineering and Natural Sciences, University of Iceland, Sæmundargötu 2, Reykjavík 101, Iceland. 5Science Institute, University of Iceland, Dunhaga 3, Reykjavík 107, Iceland.en_US
dc.identifier.journalAnalytical chemistryen_US
dc.rights.accessClosed - Lokaðen_US
dc.departmentcodeBAB12
dc.source.journaltitleAnalytical chemistry
dc.source.volume93
dc.source.issue29
dc.source.beginpage10106
dc.source.endpage10113
dc.source.countryUnited States


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