Patients with systemic lupus erythematosus are deficient in complement-dependent prevention of immune precipitation.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2004-06-01
Metadata
Show full item recordCitation
Rheumatology (Oxford). 2004, 43(6):783-9Abstract
OBJECTIVE: A functional deficiency of complement has been implicated but not conclusively demonstrated in the pathogenesis of systemic lupus erythematosus (SLE). To test this, we studied several aspects of complement in 44 patients with SLE, 46 patients with rheumatoid arthritis and 102 blood donors. METHODS: Prevention of immune precipitation (PIP) was measured by an enzyme immunoassay, levels of C1q, C4 and C3 by rocket immunoelectrophoresis, C4A, C4B and C3d by enzyme-linked immunosorbent assay (ELISA), complement haemolysis (CH50) by standard methods and C4 allotypes by high-voltage agarose electrophoresis and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: PIP was significantly reduced in SLE (P<0.001); the defect was revealed by a sensitive assay measuring this function of complement but not by the other tests employed. The patients were clinically well at the time of study, and levels of C3d, which have been shown to correlate with disease activity, were normal. The defect was more common in patients with early disease (P = 0.009), supporting a role in aetiology or early pathophysiology. PIP was positively correlated with levels of C4 (P = 3 x 10(-5)) and in particular the C4A isotype (P = 9 x 10(-10)) whereas C4B was redundant. CONCLUSIONS: Our results reveal a defect in prevention of immune precipitation in SLE that is apparent at an early stage in the disease and correlates with low levels of C4A. These results indicate that subtle deficiencies of complement may predispose to SLE.Description
To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldAdditional Links
http://rheumatology.oxfordjournals.org/cgi/content/abstract/43/6/783ae974a485f413a2113503eed53cd6c53
10.1093/rheumatology/keh183
Scopus Count
Collections
Related articles
- Defective prevention of immune precipitation in autoimmune diseases is independent of C4A*Q0.
- Authors: Arason GJ, Kolka R, Hreidarsson AB, Gudjonsson H, Schneider PM, Fry L, Arnason A
- Issue date: 2005 Jun
- Which complement assays and typings are necessary for the diagnosis of complement deficiency in patients with lupus erythematosus? A study of 25 patients.
- Authors: Boeckler P, Meyer A, Uring-Lambert B, Goetz J, Cribier B, Hauptmann G, Lipsker D
- Issue date: 2006 Nov
- C4A deficiency and elevated level of immune complexes: the mechanism behind increased susceptibility to systemic lupus erythematosus.
- Authors: Traustadottir KH, Sigfusson A, Steinsson K, Erlendsson K
- Issue date: 2002 Nov
- Complete deficiencies of complement C4A and C4B including 2-bp insertion in codon 1213 are genetic risk factors of systemic lupus erythematosus in Thai populations.
- Authors: Ittiprasert W, Kantachuvesiri S, Pavasuthipaisit K, Verasertniyom O, Chaomthum L, Totemchokchyakarn K, Kitiyanant Y
- Issue date: 2005 Aug
- Circulating immune complex levels measured by new ELISA kits utilizing monoclonal anti-C1q and anti-C3d antibodies correlate with clinical activities of SLE but not with those of RA.
- Authors: Yoshinoya S, Mizoguchi Y, Aotsuka S, Yokohari R, Nishioka K, Miyamoto T
- Issue date: 1992