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dc.contributor.authorAgnarsson, Bjorn
dc.contributor.authorIngthorsson, Saevar
dc.contributor.authorGudjonsson, Thorarinn
dc.contributor.authorLeosson, Kristjan
dc.date.accessioned2009-07-15T15:18:08Z
dc.date.available2009-07-15T15:18:08Z
dc.date.issued2009-03-30
dc.date.submitted2009-07-15
dc.identifier.citationOpt Express. 2009, 17(7):5075-82en
dc.identifier.issn1094-4087
dc.identifier.pmid19333269
dc.identifier.doi10.1364/OE.17.005075
dc.identifier.urihttp://hdl.handle.net/2336/73973
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractWe describe a new evanescent-wave fluorescence excitation method, ideally suited for imaging of biological samples. The excitation light propagates in a planar optical waveguide, consisting of a thin waveguide core sandwiched between a sample in an aqueous solution and a polymer with a matching refractive index, forming a symmetric cladding environment. This configuration offers clear advantages over other waveguide-excitation methods, such as superior image quality, wide tunability of the evanescent field penetration depth and compatibility with optical fibers. The method is well suited for cell membrane imaging on cells in culture, including cell-cell and cell-matrix interaction, monitoring of surface binding events and similar applications involving aqueous solutions.
dc.language.isoenen
dc.relation.urlhttp://dx.doi.org/10.1364/OE.17.005075en
dc.subject.meshComputer-Aided Designen
dc.subject.meshEquipment Designen
dc.subject.meshEquipment Failure Analysisen
dc.subject.meshImage Enhancementen
dc.subject.meshLensesen
dc.subject.meshMicroscopy, Fluorescenceen
dc.subject.meshRefractometryen
dc.subject.meshReproducibility of Resultsen
dc.subject.meshSensitivity and Specificityen
dc.titleEvanescent-wave fluorescence microscopy using symmetric planar waveguidesen
dc.typeArticleen
dc.contributor.departmentDepartment of Physics, Science Institute, University of Iceland, Reykjavik, Iceland.en
dc.identifier.journalOptics expressen
html.description.abstractWe describe a new evanescent-wave fluorescence excitation method, ideally suited for imaging of biological samples. The excitation light propagates in a planar optical waveguide, consisting of a thin waveguide core sandwiched between a sample in an aqueous solution and a polymer with a matching refractive index, forming a symmetric cladding environment. This configuration offers clear advantages over other waveguide-excitation methods, such as superior image quality, wide tunability of the evanescent field penetration depth and compatibility with optical fibers. The method is well suited for cell membrane imaging on cells in culture, including cell-cell and cell-matrix interaction, monitoring of surface binding events and similar applications involving aqueous solutions.


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