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dc.contributor.authorJohnston, Andrew
dc.contributor.authorSigurdardottir, Sigrun L
dc.contributor.authorRyon, Judith J
dc.date.accessioned2009-09-21T14:34:26Z
dc.date.available2009-09-21T14:34:26Z
dc.date.issued2009-08-01
dc.date.submitted2009-09-21
dc.identifier.citationCurr Protoc Immunol. 2009, Chapter 7:Unit 7.8en
dc.identifier.issn1934-368X
dc.identifier.pmid19653208
dc.identifier.doi10.1002/0471142735.im0708s86
dc.identifier.urihttp://hdl.handle.net/2336/81916
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractLocated on the inside of the throat, the paired palatine tonsils form part of the first major barrier protecting the digestive and respiratory tracts from potentially invading microorganisms. The tonsils have a surface of stratified squamous epithelium that extends into deep and branched crypts lined by reticulated epithelium, which in parts may only be one cell thick. Organized in the sub-epithelial space are B cell rich lymphoid follicles. T cells are mostly located in the extra-follicular spaces with a very high CD4:CD8 T cell ratio. In addition to the T and B cell subsets, six phenotypes of dendritic cells (DC) have been identified in the tonsils: Langerhans cells in the squamous epithelium, germinal center DC, and follicular DC in the germinal center, and another three DC phenotypes that are located in the extra-follicular area (interdigitating DC, plasmacytoid DC, and lympho-epithelial symbiosis-DC). Here, we describe the isolation of tonsil mononuclear cells from fresh human tonsil.
dc.language.isoenen
dc.publisherGreene Pub. Associates and Wiley-Interscienceen
dc.relation.urlhttp://dx.doi.org/10.1002/0471142735.im0708s86en
dc.titleIsolation of mononuclear cells from tonsillar tissueen
dc.typeArticleen
dc.contributor.departmentDepartment of Dermatology, University of Michigan, Ann Arbor, Michigan, USA.en
dc.identifier.journalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]en
html.description.abstractLocated on the inside of the throat, the paired palatine tonsils form part of the first major barrier protecting the digestive and respiratory tracts from potentially invading microorganisms. The tonsils have a surface of stratified squamous epithelium that extends into deep and branched crypts lined by reticulated epithelium, which in parts may only be one cell thick. Organized in the sub-epithelial space are B cell rich lymphoid follicles. T cells are mostly located in the extra-follicular spaces with a very high CD4:CD8 T cell ratio. In addition to the T and B cell subsets, six phenotypes of dendritic cells (DC) have been identified in the tonsils: Langerhans cells in the squamous epithelium, germinal center DC, and follicular DC in the germinal center, and another three DC phenotypes that are located in the extra-follicular area (interdigitating DC, plasmacytoid DC, and lympho-epithelial symbiosis-DC). Here, we describe the isolation of tonsil mononuclear cells from fresh human tonsil.


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